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Services
Provided by
The Genetic Engineering Services Unit (GESU)


GESU is officially conducting seed quality control for the "Seed Registration and Certification Committee - Ministry of Agriculture & Land Reclamation - Egypt" using state-of-the-art molecular markers. In addition, the following services are provided to the agricultural community:

 

I. Production of Elite Germplasm via Tissue Culture


Micropropagation of Stavia rebaudiana for
   steviosids compounds (rebaudioside) as
   a natural sweetener.

Micropropagation and maintenance of
   elite virus free germplasm in collaboration
   with international centers.

Micropropagation and conservation of
   mulberry germplasm.

 

II. Production of Diagnostic ELISA Kits for Detection of Major Phytopathogenic Viruses


  Banana Bunchy Top Virus
   (BBTV)

  Banana-Cucumber Mosaic Cucumovirus
   (Banana-CMV)

  Potato X- Potexvirus
  (PVX)

  Potato Y-Potyvirus
   (PVY)

  Tomato Yellow Leaf Curl Geminivirus
   (TYLCV)

  Zucchini Yellow Mosaic Potyvirus
  (ZYMV)

  Faba Bean Necrotic Yellows Virus
   (FBNYV)

  Bean Yellow Mosaic Potyvirus
   (BYMV)

  Broad Bean Stain Comovirus
   (BBSV)

 

III. Production of Diagnostic PCR Kits for Accurate Detection of Major Phytopathogenic Viruses


Banana Bunchy Top Virus (BBTV)
Tomato Yellow Leaf Curl Geminivirus
   (TYLCV)

 

IV. Protein Fingerprinting Using SDS-PAGE and Isozymes


Identification of genotypes, varieties and
   cultivars

Quality control of hybrid and foundation
   seed production

 

V. DNA Fingerprinting Using Molecular Markers (RFLPs, RAPDs and SSRs)


Identification and characterization purposes
   of plant and animal material

Detection of genetic variability and
   estimation of genetic relatedness

Registration of elite lines, hybrids and
   varieties for plant proprietary rights
   protection

 

VI. Custom Analytical Services

1. Identification and estimation of carbohydrates
     using HPLC

   •
plant growth regulators (indoles, gibberellins,
      cytokinins, abscisic acid)

  
sugars (monosaccharides and disaccharides)
  
vitamins (fat soluble and water soluble)
  
alkaloids, antibiotics, steroid hormones, hyaluronic
      acid, peptides indol derivatives, plant pigments,
      nucleotides and nucleosides

  
volatile alcohols, blood alcohol, aldehydes, amines,
      ketones, phenols and phenolic acids, drugs,
      perfumes, pesticides

2. Identification and estimation of fatty acids using
     GC-MS

 
fatty acids and lipids (saturated and unsaturated fatty
     acids, iodine and saponification numbers)

3. Identification and estimation of amino acids
     using Amino Acid Analyzer

     amino acids ( hydrolyzate amino acids and
      physiological amino acids)

4. Determination of total nitrogen proteins using
     Kjedal method

5. Determination of heavy metals using atomic
     absorption

     nitrogen, phosphorous, potassium, magnesium,
      calcium, sulfur, boron, molybdenum, manganese, iron,
      lead, zinc, cadmium, copper, nickel, chromium and
      cobalt

 

VII. Custom Oligonucleotide Synthesis


Now we can provide state-of-the-art cyanoethyl phosphoramidite chemistry products including custom DNA/RNA synthesis for a wide range of diverse applications in agriculture, medicine, clinical diagnostics, scientific research and others...

Because research requirements vary, we offer not only a range of products, but a selection of purification methods including reverse phase cartridge and PAGE.

 

VIII. Molecular Imaging & Densitometric Analysis


 

Establishment of GESU

Services
Provided by GESU

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