Tomatoes are grown as extensive monocultures that make it susceptible to major epidemics
such as tomato yellow leaf curl virus. Moreover, salinity intolerance is one of the most
important constraints to tomato production in Egypt. However, several sources of tolerance
have been genetically characterized, for example in the Edkawy Egyptian cultivar. A
detailed tomato genetic map has been already developed. Therefore, the main goal of this
project is to saturate the tomato map using two types of PCR-based markers to provide
evenly-spaced readily assayable markers that can exploit even low levels of sequence
divergence. These markers will be used in facilitating the introduction of resistance
genes into elite tomato background adapted to Egyptian conditions.
OBJECTIVES
• Screening selected accessions of tomato to identify RAPD
markers.
• Map these markers using segregating population.
• Develop sequence tagged sites (STSs), with PCR based
strategies, around genes of interest.
• Introgression of the resistance genes into Egyptian
commercial cultivars. |
