Genetic Characterization of Certain Ascochyta sp.
Using Random Amplified Polymorphic DNA (RAPD)

Plant diseases, especially fungal diseases, continue to be a major obstacle to the efforts which are used to elucidate the biochemical and genetic bases of the specificity and variability of fungal-plant interactions. Here we are dealing with Ascochyta leaf, pod spotting and foot rot diseases of peas.

Peas are subjected to different fungal diseases. Ascochyta leaf, pod spotting and foot rot are considered the most serious diseases that affect peas production.

OBJECTIVES

•  The identification of Ascochyta spp. (A. pisi, A. pinodes and A. pinodella) currently based on symptoms and morphological features such as size and shape of pycnidiospores and presence or absence of chlamydospores which may cause confusion in the identification.

•  RAPD assay was used as simple, rapid and reproducible technique to detect genetic variation among three species of Ascochyta and also their pathovars.

ACHIEVEMENTS

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